Figure 5.

MMEDA inhibited hypoxia-induced phospho-JNK MAP kinase and JNK mRNA expression. The effect of MMEDA (1, 10, 50 μM) on hypoxia-activated cell signaling pathways was determined by Western blotting under hypoxia stress in PC12 cells for 10 min. MMEDA effectively reduced the activation of JNK MAP kinase and caspase-3 in PC12 cells (A). PC12 cells were treated with MMEDA and subjected to hypoxia for 10 min. Levels of JNK, and β-actin mRNA were assayed by RT-PCR (B). Values represent the mean from three independent experiments. MMEDA decreased JNK mRNA as compared with that of the hypoxia. Data are expressed as mean±SEM of three independent experiments. *: P<0.05 as compared to hypoxia control