Figure 4.

Increased percentages of CD45+and CD3+ T cells in gingiva of tumor-bearing mice injected with the NK cells and fed with AJ2. Gingiva from oral tumor-bearing mice injected with NK cells and fed with and without AJ2 were harvested and single-cell suspensions were prepared as described in the Section “Material and Methods.” Percentages of CD45+ and CD3+ T cells in gingiva in four groups of mice were determined using flow cytometric analysis after staining with the respective PEcy5-conjugated and FITC-conjugated antibodies. Isotype control antibodies were used as controls (A,B).