Figure 4.

Validation of microarray data with qRT-PCR. The top 10 hub genes identified in microarray data validated by qRT-PCR is shown in bar graph. Total RNAs were isolated from aorta tissue of AAD patients and healthy donors, reverse-transcribed to cDNA and used as template for qRT-PCR analysis. Expression of each gene in aorta tissue was normalized with the respective healthy donors. CON, control healthy donor, open bars; AAD, patient with acute aortic dissection, black bars. Mean ± SEM of 6 subjects per group; ^*P < 0.05, ^**P < 0.01 (student t-test).