Table 2.
Effect of lipoic acid on regeneration frequency and number of shoots from DEC tissue
| DEC tissue age in months (from immature embryo isolation) | Regeneration frequency (%) | Number of shoots/callus | ||
|---|---|---|---|---|
| No LA in SIM and SRM | LA in SIM and SRM | No LA in SIM and SRM | LA in SIM and SRM | |
| 2 | 92.3 ± 3.05 | 97.6 ± 2.5 | 22.0 ± 3.6a | 40.0 ± 4.5b |
| 4 | 86.0 ± 2.0* | 97.0 ± 2.6* | 16.0 ± 2.0c | 40.0 ± 1.5d |
| 6 | 81.0 ± 1.0** | 94.3 ± 2.0** | 14 .0 ± 2.0e | 33.0 ± 2.5f |
Values are the means with standard deviation (SD). Different letters in the row, in calculation of number of shoots/calli is significant (P < 0.050)
* Significant at P = 0.004
** Significant at P ≤ 0.001