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. 2017 Dec 8;13:109. doi: 10.1186/s13007-017-0260-9

Table 2.

Effect of lipoic acid on regeneration frequency and number of shoots from DEC tissue

DEC tissue age in months (from immature embryo isolation) Regeneration frequency (%) Number of shoots/callus
No LA in SIM and SRM LA in SIM and SRM No LA in SIM and SRM LA in SIM and SRM
2 92.3 ± 3.05 97.6 ± 2.5 22.0 ± 3.6a 40.0 ± 4.5b
4 86.0 ± 2.0* 97.0 ± 2.6* 16.0 ± 2.0c 40.0 ± 1.5d
6 81.0 ± 1.0** 94.3 ± 2.0** 14 .0 ± 2.0e 33.0 ± 2.5f

Values are the means with standard deviation (SD). Different letters in the row, in calculation of number of shoots/calli is significant (P < 0.050)

* Significant at P = 0.004

** Significant at P ≤ 0.001