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. 2017 Dec 8;15:94. doi: 10.1186/s12958-017-0312-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Altered follicular development in TGFBR1-CA^G9Cre mice. a and b Follicle counts of control and TGFBR1-CA^G9Cre ovaries at PD5 (a) and PD7 (b). Data are mean ± s.e.m. n = 3. ^* P < 0.05. Ns, not significant. c and d Immunohistochemical localization of INHA in PD7 control and TGFBR1-CA^G9Cre ovaries. Arrows indicate abnormal follicle structures. Experiment was performed using ABC method, and signals were developed using NovaRED Peroxidase Substrate Kit. Sections were counterstained with hematoxylin. Four independent samples per group were used for immunohistochemical analyses. Scale bar is representatively depicted in (c) and equals 50 μm (c and d)