Table 1.

Promoters and terminators of the P. pastoris GoldenPiCS toolbox

Promoter/Terminator	Short Name	Gene name	ORF	length [bp]	μA mean exp. G	μA mean exp. X	μA mean exp. D	μA mean exp. M	rel. eGFP [%] G	rel. eGFP [%] X	rel. eGFP [%] D	rel. eGFP [%] M
P GAP	TDH3	glyceraldehyde-3-phosphate dehydrogenase	PP7435_Chr2–0858	496	175,734	153,293	190,970	89,048	100	100	100	100
P GPM1	GPM1	phosphoglycerate mutase	PP7435_Chr3–0360	404	118,686	98,941	165,884	52,844	25	13	26	11
P RPP1B	RPP1B	ribosomal protein P1 beta	PP7435_Chr4–0276	999	168,241	88,081	147,457	134,220	22	14	23	13
P PDC1	PDC1	pyruvate decarboxylase 1	PP7435_Chr3–1042	989	95,047	34,921	188,242	24,093	19	17	23	16
P POR1	POR1	mitochondrial porin	PP7435_Chr2–0411	704	94,576	173,321	58,699	118,862	46	28	19	51
P MDH3	MDH3	peroxisomal malate dehydrogenase	PP7435_Chr4–0136	979	26,497	206,905	12,554	87,661	76	83	17	110
P ADH2	ADH2	alcohol dehydrogenase 2	PP7435_Chr2–0821	820	117,961	165,203	140,678	61,540	33	47	16	54
P FBA1–1	FBA1–1	fructose 1,6-bisphosphate aldolase	PP7435_Chr1–0374	970	152,990	127,354	213,816	57,961	20	11	14	12
P SHB17	SHB17	sedoheptulose bisphosphatase	PP7435_Chr2–0185	995	24,473	43,866	35,852	167,426	0	0	0	53
P TEF2	TEF2	translational elongation factor EF-1 alpha	PP7435_Chr1–1535	572	184,546	96,226	180,287	161,445			53
P RPL2A	RPL2A	ribosomal 60S subunit protein L2A	PP7435_Chr4–0909	949	184,025	96,715	164,082	143,041			10
P LAT1	LAT1	dihydrolipoamide acetyltransferase	PP7435_Chr1–0349	1006	24,576	22,867	31,661	16,960			6
P PFK300	PFK300	Gamma subunit of 6-phosphofructokinase	PP7435_Chr4–0686	229	20,845	18,516	21,844	15,474			2
P GUT1	GUT1	glycerol kinase	PP7435_Chr4–0173	1305	31,172	88,114	1324	12,564	44		1
P THI11	THI11	involved in synthesis of thiamine precursor	PP7435_Chr4–0952	1005	77,578	101,948	45,756	120,542			36
P DAS2	DAS2	dihydroxyacetone synthase 2	PP7435_Chr3–0350	1005	1316	32,401	1268	186,477			0	160
P AOX1	AOX1	alcohol oxidase 1	PP7435_Chr4–0130	928	5301	159,461	4487	188,502			0	105
P DAS1	DAS1	dihydroxyacetone synthase 1	PP7435_Chr3–0352	554	2518	45,547	2200	239,261			0	99
P FDH1	FDH1	formate dehydrogenase	PP7435_Chr3–0238	1032	3126	215,618	2418	179,036			0	83
P ALD4	ALD4	mitochondrial aldehyde dehydrogenase	PP7435_Chr2–0787	869	21,239	109,237	9330	52,434			0	18
ScCYC1tt	YJR048W	cytochrome C isoform 1	Sc: YJR048W	285	–	–				100	100	100
TDH3tt	TDH3	glyceraldehyde-3-phosphate dehydrogenase	PP7435_Chr2–0858	210	175,734	153,293	190,970	89,048		139	128	126
RPS2tt	RPS2	protein component of the 40S ribosome	PP7435_Chr1–1396	476	161,620	77,215	151,802	135,407		136	129	128
RPP1Btt	RPP1B	ribosomal protein P1 beta	PP7435_Chr4–0276	507	168,241	88,081	147,457	134,220		120	117	113
RPS17Btt	RPS17B	ribosomal protein 51	PP7435_Chr2–0491	495	122,828	89,591	125,205	117,162		124	122	118
chr4_0883tt	chr4_0883	not conserved hypothetical protein	PP7435_Chr4–0069	483	127,795	93,922	95,185	113,403		88	99	84
IDP1tt	IDP1	mitochondrial NADP-specific isocitrate dehydrogenase	PP7435_Chr1–0546	503	11,349	8309	19,236	10,932		126	123	118
RPS25Att	RPS25A	protein component of the 40S ribosome	PP7435_Chr2–0346	493	146,211	100,973	140,035	126,110		140	126	123
RPS3tt	RPS3	protein component of the 40S ribosome	PP7435_Chr1–0118	174	143,928	59,278	143,257	121,372		137	125	123
RPL2Att	RPL2A	ribosomal 60S subunit protein L2A	PP7435_Chr4–0909	504	184,025	96,715	164,082	143,041		102	102	93

Mean expression from microarray (μA) data were extracted from [26]. The relative eGFP expression is related to expression under control of P_GAP in the respective condition

G 2% glycerol, X glucose feed bead (limited glucose), D 2% glucose, M methanol