Table 1. Accession numbers of the genes and primers sequences employed in quantitative RT–PCR studies.
Target gene | Accession number | Strand | Primer sequences 5′ → 3′ | Product (bp) |
---|---|---|---|---|
Caspase-3 | NM_001284409.1; NM_009810.3 | Forward | GGGCGTGTTTCTGTTTTGTT | 138 |
Reverse | TTGAGGTAGCTGCACTGTGG | |||
FasL | NM_010177.4; NM_001205243.1 | Forward | GGTCAGTTTTTCCCTGTCCA | 113 |
Reverse | CAGTGGGAGTGGTTGTGATG | |||
Fas | NM_007987.2; NM_001146708.1 | Forward | AGGCGGGTTCGTGAAACTGA | 81 |
Reverse | GGTTGGCATGGTTGACAGCA | |||
MT1 | NM_008639.2 | Forward | GAGCTGCTCAATGCCACTCA | 137 |
Reverse | ACAGGATGACCAGCAGGTTG | |||
MT2 | NM_145712.2 | Forward | GCGCACCTTGTGCATCTATC | 222 |
Reverse | GATGACAAGCAGGTTCCCCA | |||
18S | K01365.1 | Forward | CTCTGTTCCGCCTAGTCCTG | 123 |
Reverse | GGCCGTGCGTACTTAGACAT |
The two accession numbers in Caspase-3, FasL and Fas genes correspond with different splicing variants.