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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: J Allergy Clin Immunol. 2017 Jun 16;140(6):1562–1571.e5. doi: 10.1016/j.jaci.2017.05.032

Figure 2.

Figure 2

Immunofluorescence analysis of IgD+ cells in nasal tissue by using phycoerythrin-conjugated anti-IgD (red). Representative images of IgD+ cells in UT from A, control, B, CRSsNP, C, CRSwNP, and D, NP from CRSwNP patients (magnification 200x). E, IgD+ cells at the peri-glandular and F, subepithelial area in CRSsNP UT and G, peri-glandular and H, subepithelial area in NP of CRSwNP (magnification 400x). Nuclei were counterstained with 49,6-diamidino-2-phenylindole (blue). I, IgD+ cells in nasal tissue were counted semiquantitatively. J, Correlation between the numbers of IgD+ cells from immunofluorescence assay and IgD protein concentrations in matched nasal tissue. *P < .05. HPF, High-power field.