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Canonical (Nox1/NoxA1/NoxO1) and hybrid (Nox1/NoxA1/p47phox) activation of Nox1 in overexpression system using HEK cells. A: HEK cells were transfected with Nox1 and its activating subunits as indicated. ROS were measured by chemiluminescence with L012 (200 µmol/L). Stimuli-dependent activation of Nox1 was triggered by PMA (phorbol myristate acetate, 100nmol/L). B: quantification of the signal upon normalization to GFP control. n = 5, *p < 0.05 without PMA vs. with PMA.
