Table 3.

Expression profiling of NADPH oxidases and their activating subunits as determined by qPCR and MACE. Relative expression ± SEM; + p<0.05 compared to WT CTR; * p<0.05 comparing CTR and STZ of the respective knockout.

Gene:			NoxO1	p47phox	Nox1	Nox2	p67phox	p22phox	Nox4	Duox1		Duox2
MACE	WT	CTR	1 ± .45	1 ± .07	n.i.	1 ± .08	1 ± .10	1 ± .04	1 ± .09	1 ± .03		1 ± .80
		STZ	.62 ± .22	1.36 ± .25	n.i.	.95 ± .03	.82 ± .01	.78 ± .05+	1.12 ± .14		.86 ± .32	2.14 ± .41
	NoxO1-/-	CTR	n.d.	.88 ± .07	n.i.	.96 ± .08	.71 ± .01+	.91 ± .06+	.90 ± .01		.92 ± .31	1.92 ± .14
		STZ	n.d.	1.09 ± .14	n.i.	.66 ± .10	.75 ± .10	.73 ± .07+	1.06 ± .05*		.65 ± .26	.59 ± .11*
	p47-/-	CTR	.8 ± .23	n.d.	n.i.	1.29 ± .02	1.09 ± .02	.92 ± .003	.95 ± .01		.71 ± .14	2.16 ± 1.47
		STZ	.59 ± .19	n.d.	n.i.	1.61 ± .06	1.06 ± .12	.82 ± .02	1.14 ± .03*		1.56 ± .37	1.62 ± .55
	p47-/-	CTR	n.d.	n.d.	n.i.	1.11 ± .05	.81 ± .03	.88 ± .01	.94 ± .06		.58 ± .22	2.71 ± .95
		STZ	n.d.	n.d.	n.i.	1.74 ± .24	1.29 ± .12*	.97 ± .03	.98 ± .04		.39 ± .08	1.04 ± .34
qPCR	WT	CTR	1 ± .16	1 ± .41	1 ± .69	1 ± .11	n.d.	n.d.	n.d.		n.d.	n.d.
		STZ	.99 ± .11	1.87 ± .16	1.07 ± .18	n.d.	n.d.	n.d.	n.d.		n.d.	n.d.
	NoxO1-/-	CTR	n.d.	1.41 ± .11	2.02 ± .5	1.27 ± .25	n.d.	n.d.	n.d.		n.d.	n.d.
		STZ	n.d.	1.35 ± .23	1.8 ± .81	n.d.	n.d.	n.d.	n.d.		n.d.	n.d.
	p47-/-	CTR	0.78 ± .18	n.d.	2.8 ± .45	1.39 ± .13	n.d.	n.d.	n.d.		n.d.	n.d.
		STZ	0.54 ± .11+	n.d.	2.42 ± 1.08	n.d.	n.d.	n.d.	n.d.		n.d.	n.d.
	O1/47-/-	CTR	n.d.	n.d.	n.d.	1.67 ± .17	n.d.	n.d.	n.d.		n.d.	n.d.
		STZ	n.d.	n.d.	n.d	n.d.	n.d.	n.d.	n.d.		n.d.	n.d.