FIGURE 7.

Minimal inhibitory bacitracin concentration of bcrC and uppP deletion and complementation mutants. Strains (W168, TMB297, TMB3694, TMB3739, TMB3408, TMB3695, and TMB3740) were inoculated from fresh overnight cultures (xyl+) in MCSEC at 37°C with or without 0.2% xylose. During exponential growth, cells were embedded in soft agar and plated as an overlay on MCSEC agar. One Etest^® strip (bacitracin 0.016–256 μg ml^-1) was placed on the soft agar (see Material and Methods). The MIC was determined after 24 h of incubation at 37°C. Data is shown for at least three independent biological replicates (two replicates, if MIC > 256 μg ml^-1) with mean and standard deviation. The full data set is depicted in Supplementary Figure S7.