Table 6.
CRISPR/Cas elements identified in bacterial host genome sequences.
| Bacterial host strain | Cas proteins* | DR (no. repeats; repeat length), nt co-ordinates** | DR homology (10−3 e-value threshold)*** | Spacers (no.) | Spacer sequence homology (10−3 e-value threshold)**** |
|---|---|---|---|---|---|
| Bacteriodes sp. AR20 | None | DR1 (4, 23 nt) contig 52.52 nt 609–778 | No homology | 3 | R. albus plasmid pAR67 (D88665.1) |
| R. albus AR67 | None | DR1 (4; 24 nt) contig1.1 nt 261160–261418 | No homology | 3 | No homology |
| DR2 (4, 24 nt) contig 1.1 nt 261550–261807 | No homology | 3 | No homology | ||
| DR3 (4, 24 nt) contig 14.14 nt 9638–9973 | No homology | 4 | No homology | ||
| DR4 (12, 23 nt) contig 31.31 nt 1118–1998 | No homology | 11 | No homology | ||
| DR5 (4, 24) contig 44.44 nt 14202–14460 | No homology | 3 | No homology | ||
| S. equinus 2B | Cas2, Cas1, Csn1 | None | |||
| S. equinus Sb04 | Csn1, Cas1, Cas2, Csn2, Cas Csm6 | DR1 (21; 36) contig 101.101 nt 253157–254514 | S. thermophilus strain ND07 (CP016394.1); S. thermophilus strain KLDS (CP016877.1); S. thermophilus strain KLDS SM (CP016026.1) | 20 | No homology |
| S. equinus Sb17 | Cas Csm6 | None |
*
CRISPR associated (Cas) proteins from IMG genome annotation;
**
DR with >3 repeats;
***
DR homology, top 3 best hits results, BLASTn against the NCBI nr database (January 2017 update), e-value threshold of 10−3.
****
Spacer sequence homology top 3 best hits results, BLASTn against the NCBI nr database (January 2017 update), e-value threshold of 10−3.