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. 2017 Dec 5;8:2393. doi: 10.3389/fmicb.2017.02393

FIGURE 5.

FIGURE 5

Detection of the P. parvulus 2.6 gut operon by Southern blot hybridization. Plasmids preparations of the P. parvulus strains were fractionated by electrophoresis in agarose gel, transferred to membranes and hybridized for detection of gutF, gutR and gutB with the probes 1, 2, and 3, respectively. (A) Physical map of the gut operon, including the putative promoter of the gut operon (Pgut), the operon genes (gutF, gutR, gutM gutC, gutB and gutA), a secondary structure including the 3′-end of gutR and the 5′-end of gutM (coordinates 3483-3512 in GenBank accession No MF766019) as well as a transcriptional terminator (coordinates 6103-6027 in GenBank accession No MF766019) downstream of gutA. In addition, location of probes 1, 2, and 3 is indicated. (B) Images of the corresponding gels and hybridized membranes. The double headed arrows indicate the position of the hybridized bands in the corresponding gel.