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. 2017 Dec 5;8:2393. doi: 10.3389/fmicb.2017.02393

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Copyright © 2017 Pérez-Ramos, Werning, Prieto, Russo, Spano, Mohedano and López.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Trans complementation of the sorbitol utilization regulatory machinery in L. plantarum 90. Bacteria carrying (A,E,F) pRCR16 (, ), (B,E,F) pRCR17 (, ), (C,E,F) pRCR18 (, ) or (D,E,F) pRCR19 (, ) were grown in MRSG and at time 0 were transferred to fresh MRSS (open symbols) or to MRSG (closed symbols). OD_600nm and fluorescence of the cultures were monitored every hour. Overlays of images of the cultures taken at time 0 and after 2, 4, and 6 h incubation by phase contrast and fluorescent microscopy are depicted. The experiments were performed in triplicate and the mean value and standard deviation is depicted.

Inline graphic — Trans complementation of the sorbitol utilization regulatory machinery in L. plantarum 90. Bacteria carrying (A,E,F) pRCR16 (, ), (B,E,F) pRCR17 (, ), (C,E,F) pRCR18 (, ) or (D,E,F) pRCR19 (, ) were grown in MRSG and at time 0 were transferred to fresh MRSS (open symbols) or to MRSG (closed symbols). OD_600nm and fluorescence of the cultures were monitored every hour. Overlays of images of the cultures taken at time 0 and after 2, 4, and 6 h incubation by phase contrast and fluorescent microscopy are depicted. The experiments were performed in triplicate and the mean value and standard deviation is depicted.