Table 2.
Oligonucleotides used in this work.
| Primers | Sequence (5′- 3′) | Utilization | Amplicon size (bp) |
|---|---|---|---|
| pts1F | TGCGGAAGCGGTTAATCGGCT | gutF DNA probea | 618 |
| pts1R | CCACGACTCTTGCCTCCCGCA | ||
| ptsRF | CGAACTGGAAGCAACCTGGGA | gutR DNA probea | 652 |
| ptsRR | CCGATGAATAATTGGCGCTGC | ||
| ptsBF | GGAATGGAAGCTGTTGATGGC | gutB DNA probea | 643 |
| ptsBR | CAACGCCAATCAAGGTCCCGA | ||
| pgutBglIIF | GAAGATCTACCATATGGCGATAATGAAAA | Cloning of Pgut in pRCRa | 186 |
| pgutXmaIR | TCGCTCCCGGGTCATTTCTTTTC | ||
| gutRXmaIF | CGTGGTTAACCCGGGAATTTTAGTTG | Cloning of gutR in pRCR16a | 1952 |
| gutRXbaIR | GCTCTAGAAACGCACTGACTAGGATCA | ||
| gutMXmaIF | TCCCCCCGGGTTAAATCAGTTGATGGA | Cloning of gutM in pRCR16a | 597 |
| gutMXbaIR | GCTCTAGAACAGCCCATAAAGCCC | ||
| gutRXmaIF | CGTGGTTAACCCGGGAATTTTAGTTG | Cloning of gutRM in pRCR16a | 2472 |
| gutMXbaIR | GCTCTAGAACAGCCCATAAAGCCC | ||
| pPP1∗F | CATAGTTCACTGGGCTACCA | Sequencing of pPP1∗b | – |
| pPP1∗R | TAGCGGTGCCTCCCTTTAAT | – |
aPlasmidic DNA preparations of P. parvulus 2.6 was used as substrate for the PCR reactions. bPlasmidic DNA preparations of P. parvulus 2.6NR was used as substrate for the DNA sequencing.