Table 4.

Heterologous expression of components of P. parvulus 2.6 gut operon in L. plantarum 90 carrying pCRC derivatives plasmids.

L. plantarum strains	DNA insert in pRCR derivatives	Medium	Initial growth ratea (μ)	Sorbitol induced growth rateb (μ)	Specific fluorescencec
					2 h	3 h	4 h	5 h	6 h
90[pRCR16]	Pgut	MRSS	0.416 ± 0.045	0.251 ± 0.034	1.48 ± 1.03	5.86 ± 1.43	10.42 ± 1.56	13.44 ± 0.51	14.81 ± 0.66
90[pRCR17]	Pgut-gutR	MRSS	0.455 ± 0.065	0.259 ± 0.020	4.42 ± 1.22	13.41 ± 0.90	22.38 ± 2.02	20.30 ± 1.62	17.28 ± 1.65
90[pRCR18]	Pgut-gutM	MRSS	0.429 ± 0.025	0.239 ± 0.048	1.62 ± 0.69	10.26 ± 2.30	18.05 ± 2.85	24.26 ± 1.51	26.83 ± 1.83
90[pRCR19]	Pgut-gutRM	MRSS	0.495 ± 0.011	0.256 ± 0.016	2.80 ± 0.77	11.74 ± 1.51	19.26 ± 2.10	17.20 ± 1.71	15.98 ± 1.21
90[pRCR16]	Pgut	MRSG	0.889 ± 0.059	ND	0.06 ± 0.03	0.04 ± 0.00	0.04 ± 0.00	0.04 ± 0.01	0.04 ± 0.01
90[pRCR17]	Pgut-gutR	MRSG	0.825 ± 0.029	ND	0.07 ± 0.06	0.06 ± 0.02	0.04 ± 0.01	0.03 ± 0.00	0.03 ± 0.02
90[pRCR18]	Pgut-gutM	MRSG	0.803 ± 0.049	ND	0.06 ± 0.03	0.03 ± 0.03	0.03 ± 0.01	0.04 ± 0.01	0.03 ± 0.01
90[pRCR19]	Pgut-gutRM	MRSG	0.832 ± 0.034	ND	0.04 ± 0.02	0.03 ± 0.02	0.03 ± 0.01	0.04 ± 0.02	0.03 ± 0.02

^aGrowth rate of the cultures grown in MRSS was calculated from the OD_600nm obtained during the first 2 h of induction. ^bGrowth rate of the cultures grown in MRSS was calculated from the OD_600nm obtained during the 2–6 h of induction, besides for 90[pRCR18], that due to the stalling of growth from 2 h to 3 h of incubation the growth rate was calculated from the data obtained from 3 h to 6 h of incubation. ND, the growth rate was not determined because the cultures have entered in the stationary phase of growth. ^cSpecific fluorescence was calculated as the ratio of the detected fluorescence (5x) and the bacterial biomass estimated from the OD₆₀₀nm of the culture.