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. 2017 Dec 5;8:2071. doi: 10.3389/fpls.2017.02071

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Copyright © 2017 An, Lan, Deng, Chen, Ouyang, Shi, Yang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Catalytic properties of the recombinant OsD-LDH-FL. (A) Substrate specificity screening of OsD-LDH, performed at 25°C and pH 8.35 using Cyt c as the electron acceptor. (B) Electron acceptors screening of OsD-LDH, performed at 25°C and pH 8.35. (C) pH optimum of OsD-LDH, performed at 25°C using PMS-DCIP as the electron acceptor. (D) Temperature optimum of OsD-LDH, performed at pH 8.35 using PMS-DCIP as the electron acceptor. (E–H) Kinetic parameters of OsD-LDH toward D-lactate and D-2-HB using PMS-DCIP and Cyt c as electron acceptors, respectively; V_max and K_M were determined using a Hanes–Woolf plot method and all experiments were performed at 30°C and pH 8.35. D-2-HB, D-2-hydroxybutyrate; D-Lac, D-lactate; L-Lac, L-lactate; Glyce, Glycerol; Glyco, Glycolate; 3-HB, 3-hydroxybutyrate; GST, Purified Glutathione S-transferase as negative control. Values represent the mean ± SD (n = 3).