Figure 2.

Vision Analysis following Subretinal AAV5-VMD2-CR2-fH Injection

(A and B) Optokinetic response was recorded before injection and one month following injection. (A) By measuring the spatial frequency threshold at a constant speed (12°/s) and contrast (100%), visual acuity was measured (n = 14–17 mice per condition). (B) To measure contrast sensitivity, the reciprocal of the contrast threshold at a fixed spatial frequency (0.131 cycles per degree) and speed (12°/s) were calculated (n = 12–18 mice per condition). (C) ERG c-wave amplitudes were measured using full-field electroretinography one month following subretinal injection (n = 12–14 mice per condition; ***p ≤ 0.001; NS, not significant, p = 0.65). (D) Focal ERG response was recorded at 3 flash strengths for a and b waves (D). ERG analysis for areas proximal to and distal from the injection (injury) were compared to non-injected eyes (n = 10 animals per group). Data are expressed as mean ± SEM.