Figure 4.

Immunoblot-Based Demonstration of the Involvement of p-ERK, pp38, p-AKT, Cleaved Caspase-9, Cleaved Caspase-3, Cleaved PARP, and Cytochrome c in 2-hr PC-SA-Treated Cells

(A–D) To check the involvement of signaling molecules in PC-SA-mediated apoptosis, (A) U937, (B) K562, (C) B16F10, and (D) RAW264.7 cells were treated with varying concentrations of PC-SA. The whole-cell lysates were subjected to western blot with anti-p-ERK, anti-pp38, anti-p-AKT, anti-cleaved caspase-3, anti-cleaved caspase-9, and anti-cleaved PARP antibodies. (E) Cytosolic fractions were subjected to western blot with anti-cytochrome c antibodies. Anti-β-actin antibodies were used to verify equal amounts of protein loading in each well.