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. 2017 Dec 5;8:2054. doi: 10.3389/fpls.2017.02054

FIGURE 2.

FIGURE 2

Microscopic structure of embryogenic and non-embryogenic tissues induced from immature embryos and suspension cultures in Chinese fir. Stage annotation based on Filonova et al. (2000). (A) Embryogenic tissues. Cell aggregates include a clump of rounded, dense cytoplasmic cells surrounded by a set of vacuolated cells that are elongated. (B) Well-organized PEM III structures after a more than half year maintenance period. (C,D) Non-embryogenic tissues also induced from immature embryos of Chinese fir: cell clusters composed of dense cytoplasmic cells but lacking elongated cells (C), or with vacuolated cells in a collapsed cell shape but stuck in dense cytoplasmic cell aggregates (D, arrow represented PEM I structure). (E,F) Suspensions of PEMs stained with acetocarmine and Evan’s blue: (E) PEMs in suspension for 1 week, with more early-stage PEMs dedifferentiated (PEM I) (arrows); (F) PEMs in suspension for 3 weeks, with newly organized PEMs. Bars = 100 μm.