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(A) Single-shot 15N NMR spectrum of the fully deuterated substrate (0.125 M) solution in D2O obtained after 30 s of p-H2 bubbling (50% p-H2 fraction) and polarization transfer to 15N using the magnetic shield. (B) Same as (A) but with the protonated substrate (0.125 M). (C) Same as (B) but with an 80% p-H2 fraction.
