Extended Data Figure 4. Analysis of mouse development by mmPCR–seq.

a, Comparison of average editing levels between mouse brain and liver at mid-embryogenesis stage E12.0–E13.0 (n =4 biological replicates). b, Comparison of RNA editing between mouse brain and liver. At mid-embryogenesis (E12.0–E13.0), most sites are edited at higher levels in the liver than in the brain. However, as development progresses over time (postnatal 2 days and 6 months), the brain becomes the dominant tissue of editing activity instead. c, Heat map of editing levels in mouse liver and brain during development. We observed an overall trend of increased editing over development in brain. d, Sanger validation of two editing sites in the mouse Cacna1d gene that show an increase in editing levels over development. e, A total of 30 sites, in which the editing levels remained stable over development, including the Gria2 Q/R site. These sites were required to have an average editing within the 75th percentile and no significant increase or decrease in editing over development (P >0.02, F-test, and slope <0.01, linear regression). f, Sanger validation of one site in the Copa gene that showed constant editing levels over mouse brain development. g, Average editing levels in different mouse tissues over development. h, ADAR expression levels in different mouse tissues over development.