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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Curr Opin Chem Biol. 2017 Oct 20;41:20–27. doi: 10.1016/j.cbpa.2017.10.002

Figure 2.

Figure 2

Structural changes induced by the lack of KsgA-mediated methylation in T. thermophilus 30S ribosomal subunit. Methylation of A1518 and A1519 is necessary for the formation of a hydrogen-bonding network between two helices, and stabilization of kasugamycin’s binding site. (A) Crystal structure of the wild-type, fully methylated 30S subunit (PDB 1J5E). Resolution of the structure (3.05 Å) prevented modeling of methylation. (B) Crystal structure of the 30S subunit from ΔksgA strain (PDB 3OTO).