Skip to main content
. Author manuscript; available in PMC: 2018 Nov 28.
Published in final edited form as: J Control Release. 2017 Oct 4;266:321–330. doi: 10.1016/j.jconrel.2017.10.002

Figure 6. Effect of the released MK2i from PCL–HT/MK2i substrate on phosphorylation of HSP27 and CREB in VSMCs.

Figure 6

(a) Representative images of western blots showing the phosphorylation of target proteins (pHSP27 and pCREB) in VSMCs treated with the released MK2i. The PCL–HT/MK2i samples were incubated in low serum growth medium (1% FBS), and the extract solutions were collected after 6 h, 1, 3 and 7 days. Following treatment of each extract to VSMCs for 2 h, LPA (30 μM) was exposed for 30 min. As a positive and negative control, the medium with or without 200 μg/mL of MK2i was used. (b) Quantification of the relative ratios (phosphor-HSP27/total HSP27 and phosphor-CREB/total CREB). Results are shown as the average values ± S.D. (n = 3; *P < 0.05 compared to NT).