Fig. 6.

Transient expression analysis of BjPR1 promoter in tobacco leaves: a schematic representation of BjPR1 promoter cloned in pORER2 vector (promoter less GUS reporter vector) at Pst1 and BamH1 sites for studying promoter inducibility; b healthy N. benthamiana plants for transient expression analysis. After 24 h of agroinfiltration, plants were treated with sterile water (control), ABA, JA, and SA, respectively. Wounding was carried out with sterile needle. Leaf samples were harvested from control and treated plants after 24 h of treatment for GUS staining; c Promoter less GUS reporter vector as negative control; d GUS gene expression driven by BjPR1 promoter without treatment; e effect of ABA on the expression of GUS gene driven by BjPR1 promoter; f effect of JA on the expression of GUS gene driven by BjPR1 promoter in tobacco leaf; g Effect of SA on the expression of GUS gene driven by BjPR1 promoter; h wound-induced GUS accumulation in tobacco leaf