Table 3.

Results of histological examination of formalin‐fixed, ethanol‐preserved museum specimens

Institution	Collection #	Species	County	Year	qPCR replicates positive	Histology result
CAS	39865	Anaxyrus boreas	Los Angeles	1915	2	Lesions of hyperplasia and hyperkeratosis, no Bd organisms
CAS	39867	A. boreas	Los Angeles	1915	3	Lesions of hyperplasia and hyperkeratosis, no Bd organisms
CAS	63049	Hyliola cadaverina	Ventura	1927	2	Negative
MVZ	27881	Rana catesbeiana	Los Angeles	1939	3	Negative
MVZ	33668	Rana boylii	Ventura	1940	2	Negative
MVZ	33672	R. boylii	Ventura	1940	2	Negative
LACM	13455	Rana draytonii	Los Angeles	1947	2	Negative
LACM	13703	R. boylii	Ventura	1954	2	Negative
LACM	13496	R. draytonii	Ventura	1954	2	Negative
CAS	181067	A. boreas	Santa Barbara	1961	2	Negative
LACM	13457	R. draytonii	Los Angeles	1963	2	Negative
CAS	190944	Anaxyrus californicus	Santa Barbara	1966	2	Negative
LACM	76274	R. draytonii	Ventura	1968	3	Negative
Private	NA	A. californicus	Santa Barbara	1989	1	Hyperplasia and hyperkeratosis, no Bd organisms
Private	NA	R. boylii	San Benito	1989	0	Hyperplasia and hyperkeratosis with Bd organisms
Private	NA	R. boylii	San Benito	1989	0	Hyperplasia and hyperkeratosis, no Bd organisms
Private	NA	R. boylii	San Benito	1989	0	Hyperplasia and hyperkeratosis, no Bd organisms
Private	NA	R. boylii	San Benito	1989	0	Hyperplasia and hyperkeratosis, no Bd organisms
Private	NA	R. boylii	San Benito	1989	0	Hyperplasia and hyperkeratosis, no Bd organisms
Private	NA	R. boylii	San Benito	1989	0	Hyperplasia and hyperkeratosis, no Bd organisms