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. 2017 Dec 6;8:204. doi: 10.3389/fgene.2017.00204

FIGURE 1.

FIGURE 1

Differential response of plasma corticosterone (CORT) following acute, chronic immobilization stress (CS) or early-life nutritional stress in birds. (A) Attenuation of CORT response in chronic stressed birds compared to acute stressed birds by immobilization. Plasma CORT concentrations (pg/ml) were measured by radioimmunoassay (RIA) after acute or CS (n = 7–8 birds/group). (B) Plasma CORT concentrations were measured by RIA (n = 18 birds/group, duplicate). Treatments of 12 h (Day 1) or 36 h (Day 2) FD after post-hatching, blood was sampled from 0 h controls (Con), 12 h fed controls (12hF), 12 h FD (12hFD), 36 h fed controls (36hFD), and 36 h FD birds (n = 14birds/group). (C) At 6 weeks of age, blood was sampled from birds which were treated by 12hFD and 36hFD following post-hatching (Days 1 and 2). Plasma CORT concentrations were measured by RIA. Data (mean ± SEM) are presented as the fold changes of relative expression levels compared to the unstressed control group (Con) set at a value of 1.0. Different lower-case letters above the bars denote significant differences (P < 0.05) among groups, where a > b > c and b,c is not different from b or c.