Table 1.

Primers used for RT-qPCR.

Gene	GenBank#	Primer sequences (5′–3′)	Size (bp)	Annealing temperature (°C)
DNMT1	NM_206952	F:CATCCTCAGGGACCACATCT	169	58
		R: CTTCTTCTCGTGGTGGGTGT
DMAP1	XM_004936716	F: CTTTGTGCTGCCTACTGCTG	151	58
		R: AGTTCTGCTCCTTGCTTTGC
DNMT3A	NM_00102483223	F: CACCACTCGCTCCAACTCCA	141	60
		R: ATGTTGGACACGTCCGTGT
DNMT3B	NM_00102482833	F: CTGCACACAGAGCTCGCTAC	167	60
		R:GAGAGGGGAGAAGAGGTGCT
GR	NM_001037826	F: GCCATCGTGAAAAGAGAAGG	95	54
		R: TTTCAACCACATCGTGCAT
GAPDH	NM_204305	F: CTTTGGCATTGTGGAGGGTC	128	58–60
		R: ACGCTGGGATGATGTTCTGG
β-actin	L08165	F: CACAATGTACCCTGGCATTG	158	54–56
		R: ACATCTGCTGGAAGGTGGAC