FIGURE 3.

Envelope cholesterol is critical for HSV-1 mediated cell fusion. MβCD-treated or mock-treated HSV-1 ANGpath was diluted in culture medium and added to Vero cells for 4 h in the presence of cycloheximide. Cells were fixed and stained. Micrographs of fusion-from-without (FFWO) were captured (A) and quantitated (B) as described in Section “Materials and Methods.” The results shown are representative of three independent experiments.