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. 2017 Dec 6;8:1716. doi: 10.3389/fimmu.2017.01716

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Copyright © 2017 Hellmann, Lother, Wurster, Lutz, Schmitt, Morton, Eyrich, Czakai, Einsele and Loeffler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Human dendritic cells (DCs) contact and phagocytose Aspergillus fumigatus conidia and germ tubes more effectively. Representative images show phagocytosis of red fluorescent conidia and germ tubes by human (A) and murine (B) DCs at the indicated time points (duration of coculture). Number of touches (C) of human and murine DCs with A. fumigatus conidia (con), germ tubes (gt), and polystyrene beads (beads) and phagocytosis rates (D) were determined by live imaging analysis. Mean values of touched (C) or mean percentage of phagocytosed (D) fungal cells or beads after 3 h of coculture are given in the figure. Six human and murine donors were assessed. Significance was calculated with unpaired t-Test and error bars indicate SDs.