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. 2017 Dec 6;8:1716. doi: 10.3389/fimmu.2017.01716

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Copyright © 2017 Hellmann, Lother, Wurster, Lutz, Schmitt, Morton, Eyrich, Czakai, Einsele and Loeffler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Human and murine dendritic cells show inverse Dectin-1 surface expression patterns after exposure to Aspergillus fumigatus. Dectin-1 expression on the cell surface of human moDCs (A) and murine bone marrow derived dendritic cells (BMDCs) (B) was analyzed by flow cytometry after coculture with A. fumigatus conidia and germ tubes for different periods. Mean fold changes (±SDs) of mean fluorescence intensity (MFI) compared to unstimulated cells are given in the figure. Two murine and four human samples were analyzed. The lower panels directly compare Dectin-1 expression following exposure of human moDCs and murine BMDCs to A. fumigatus germ tubes (C) and conidia (D). Human and murine samples were compared with unpaired t-Test, error bars show SDs.