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. 2017 Dec 6;8:1716. doi: 10.3389/fimmu.2017.01716

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Copyright © 2017 Hellmann, Lother, Wurster, Lutz, Schmitt, Morton, Eyrich, Czakai, Einsele and Loeffler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stronger upregulation of maturation markers and major histocompatibility complex (MHC) molecules upon Aspergillus fumigatus stimulation is observed in human moDCs. Surface expression of CD80 (A), CD86 (B), MHC-I (C), and MHC-II (D) was analyzed by flow cytometry in human moDCs and murine bone marrow derived dendritic cells after 24 h stimulation with A. fumigatus conidia (con) and germ tubes (gt) as well as depleted zymosan (dZym) and LPS. Six human and murine dendritic cell samples were assessed. Mean fold changes of MFI values compared to unstimulated cells (unst) and SDs are shown. Significance between human and mouse specimen was calculated with the unpaired t-Test, the comparison to unstimulated specimens is shown as stars above bars and was calculated with ANOVA and Tukey post-test.