FIGURE 2.

Histochemical GUS staining of developing seeds of α-amylase promoter-GUS plants. Two independent lines of T2 transgenic plants harboring respective α-amylase promoter-reporter chimeric genes were ripened as described in the legend of Figure 1 (NT and HT for 27°C/22°C and 33°C/28°C plots, respectively), and the developing T3 seeds were sampled at the indicated DAF. Longitudinal sections stained for 22 h (α-amylases, Wx [granule-bound starch synthase I], and vector control [VC]) or 30 min (Pro10 [10-kDa prolamin] and GluB1[glutelin B1]) are shown. The mature grains of Nipponbare WT, which were ripened under the same conditions, are shown in the right lower panel. The bar indicates 1 cm.