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. 2017 Nov 27;13(12):1540–1546. doi: 10.7150/ijbs.23000

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Adipocyte and osteoclast formation was increased in β-catenin^Col2CreER KO mice. (A and B) Histologic analysis showed that significant bone loss and accumulation of adipocytes in bone marrow stromal (BMS) cells in 3-month-old β-catenin^Col2CreER KO mice. (C) TRAP staining results showed that TRAP-positive osteoclasts (indicated by black arrowheads) and adipocytes (indicated by yellow arrowheads) were significantly increased in 3-month-old β-catenin^Col2CreERKO mice. (D-F) Results of histomorphometric analysis showed that bone volume (%, BV/TV) and osteoblast numbers (N.Ob) were significantly reduced and osteoclast numbers (N.OC) was significantly increased in β-catenin^Col2CreER KO mice. (G and H) We isolated BMS cells from 3-month-old β-catenin^Col2CreER KO and Cre-negative mice and cultured these cells with adipocyte differentiation medium for 3 days. Results of Oil red O staining and expression of adipocyte marker genes, including PPAR-γ and cEBP-α, showed that adipocyte formation was increased in β-catenin^Col2CreER KO mice. (I and J) We further isolated BMS cells from β-catenin^flox/flox mice and infected these cells with Ad-CMV-Cre or Ad-CMV-GFP (control virus). β-catenin levels were significantly reduced and expression of adipocyte marker genes was significantly increased in β-catenin deficient BMS cells.

Adipocyte and osteoclast formation was increased in β-catenin^Col2CreER KO mice. (A and B) Histologic analysis showed that significant bone loss and accumulation of adipocytes in bone marrow stromal (BMS) cells in 3-month-old β-catenin^Col2CreER KO mice. (C) TRAP staining results showed that TRAP-positive osteoclasts (indicated by black arrowheads) and adipocytes (indicated by yellow arrowheads) were significantly increased in 3-month-old β-catenin^Col2CreERKO mice. (D-F) Results of histomorphometric analysis showed that bone volume (%, BV/TV) and osteoblast numbers (N.Ob) were significantly reduced and osteoclast numbers (N.OC) was significantly increased in β-catenin^Col2CreER KO mice. (G and H) We isolated BMS cells from 3-month-old β-catenin^Col2CreER KO and Cre-negative mice and cultured these cells with adipocyte differentiation medium for 3 days. Results of Oil red O staining and expression of adipocyte marker genes, including PPAR-γ and cEBP-α, showed that adipocyte formation was increased in β-catenin^Col2CreER KO mice. (I and J) We further isolated BMS cells from β-catenin^flox/flox mice and infected these cells with Ad-CMV-Cre or Ad-CMV-GFP (control virus). β-catenin levels were significantly reduced and expression of adipocyte marker genes was significantly increased in β-catenin deficient BMS cells.