Figure 4.

O₂ supplementation increased the tibia parameters of Tibetan chickens. (A) Standard for the measurement of T. length (tibia length) and T. mid-diameter (tibia mid-diameter). (B) Correlation analysis of BW and T. weight during the experiment with Pearson test. (C-F) The weight, length, and mid-diameter of the tibia were record and the tibia weight index (T. weight index) was determined as the tibia weight per body weight (T. weight/BW, mg/g) between OSG and NHG. (g) The change of tibial parameters were shown in heat map using the indicated pseudo color scale from percent 0 (green) to percent 100 (red) relative to average values. The data are presented as the mean ± SD. Student's t test, ^*p<0.05, ^***p<0.001, n=16 tibia samples; ns: not significant; NHG: natural hypoxia group; OSG: oxygen supplementation group.