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Primers used for qRT-PCR analysis
| Gene | Primer Sequence (5'-3') | Amplicon size | Genbank |
|---|---|---|---|
| GAPDH | Forward: CCTCTCTGGCAAAGTCCAAG Reverse: GGTCACGCTCCTGGAAGATA |
176 bp | NM_204305.1 |
| HIF-1ɑ | Forward: TGAGAGAAATGCTTACACACAG Reverse: TGATGGGTGAGGAATTGGTTCAC |
263 bp | XM_015287266.1 |
| VEGFA | Forward: CGATGAGGGCCTAGAATGTGTC Reverse: AGCTCATGTGCGCTATGTGC |
101 bp | NM_205042.2 |
| VEGFR1 | Forward: TGTAACTAAGTATGCCTGTGG Reverse: GGAGTTGTTGGGTATCTGC |
114 bp | NM_204252.1 |
