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. 2017 Dec 6;8:2066. doi: 10.3389/fpls.2017.02066

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Copyright © 2017 Sun, Mei, Deng, Xiong, Sun, Zhang, Wen, Liu, You, Nasrullah, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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High accumulation of the ACS7 protein promotes leaf senescence in Arabidopsis. (A) The 25-day-old GVG:GUS, GVG:ACS7^Δ1-14-Flag and GVG:ACS7-Flag transgenic Arabidopsis plants were sprayed daily with either 30 μM DEX (+DEX) or its solvent, ethanol (+mock), for 3 days. The rosettes were photographed at an additional 4 days. (B) The expressions of several senescence-associated marker genes were up-regulated in the leaves of GVG:ACS7^Δ1-14-Flag after dark treatment. The 25-day-old GVG:GUS, GVG:ACS7^Δ1-14-Flag and GVG:ACS7-Flag transgenic Arabidopsis plants were sprayed with 30 μM DEX and 24 h later the fifth and sixth leaf of each were harvested for RNA extraction. The expression levels of NAP1, WRKY6, and SAG113 were determined by quantitative RT-PCR analysis with TIP41-like used as an internal control; and the relative value of each gene under mock treatment were normalized as 1. (C) The 25-day-old GVG:GUS, GVG:ACS7^Δ1-14-Flag, and GVG:ACS7-Flag transgenic Arabidopsis plants were sprayed with either 30 μM DEX (+DEX) or its solvent, ethanol (mock), and the fifth leaves were harvested after 24 h for protein extraction. Accumulation levels of ACS7-Flag and ACS7^Δ1-14-Flag were detected using immunoblot with Coomassie brilliant blue staining as the loading control. In total three and two independent transgenic lines were obtained for GVG:ACS7-Flag and GVG:ACS7^Δ1-14-Flag, respectively, and data shown here were representatives of the typical results from three independent biological replicates. Error bars represent SD in all cases.