FIGURE 5.
Comparisons of changes in the promoter activity and protein accumulation of ACS7 during leaf development. The light-grown ACS7:N7(1-54)-GUS and ACS7:GUS transgenic Arabidopsis with approximately the same transcription levels of the GUS gene were photographed at six different stages including stage 0.5, 0.7, 1.02, 1.04, 5.10, and 6.00, respectively, as described in Boyes et al. (2001). The GUS staining method was described in section “Materials and Methods.” Three to six independent biological replicates were carried out for each transgenic line to give the typical results presented.