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Sup35 is cleaved at the N-terminal prion domain. A, YCplac22-SUP35-Flag(C-tag) (yAO149) and YCplac22-Flag-SUP35(N-tag) (yAO150) transformants were cultured in YPDA after a 12-h starvation in YPA. Cells were harvested at the specified time points and analyzed by Western blotting (WB) using anti-FLAG. B, schematic representation of Sup35. The N-terminal prion domain (1–124) consists of a Q/N-rich stretch and oligopeptide repeats region. Cleavage occurs at Ala39.
