Figure 3.

Glucose-producing and CAC-related fluxes in sedentary and treadmill-running mice lacking liver AMPKα. A, schematic representation of select glucose-producing and CAC-related fluxes for ²H/¹³C metabolic flux analysis. Shown is a time course of plasma NEFAs (B) and arterial blood glucose concentrations in WT mice and mice exhibiting a liver-specific deletion of AMPK α1 and α2 subunits (KO) (C). Shown are model-estimated, absolute nutrient fluxes (μmol kg⁻¹ min⁻¹) in WT and KO mice at rest and during 30 min of treadmill running at 45% of maximal running speed: V_EndoRa (D), V_PYGL (E), V_Aldo (F), V_GK (G), V_Enol (H), V_PK+ME (I), V_LDH (J), V_PC (K), V_PCK (L), V_CS (M), V_PCC (N), and V_SDH (O). Data are mean ± S.E. (error bars); n = 6–9 mice/group. *, p < 0.05 versus WT at a specified time point. †, p < 0.05 versus 0-min time point within group.