Figure 5.

Metabolites in mice lacking hepatic AMPKα following exercise. Livers from WT mice and mice exhibiting a liver-specific deletion of AMPK α1 and α2 subunits (KO) were obtained from catheterized mice at the 30-min time point following completion of the stable isotope infusions and treadmill running bout (45% of maximal running speed). A, liver AMPKα as determined by immunoblotting and representative immunoblot of hepatic AMPKα and β-actin. B, liver glycogen content (mg g⁻¹). C, energy charge ([ATP + 0.5 ADP]/[TAN]). Shown are hepatic adenine nucleotides ATP (D), ADP (E), AMP (F), and total adenine nucleotide pool (TAN = ATP + ADP + AMP; μmol g⁻¹) (G). Shown are liver lipids (μg mg⁻¹), including triglycerides (H), diglycerides (I), phospholipids (J), and cholesterol esters (K). Data are mean ± S.E. (error bars); n = 6–9 mice/group. *, p < 0.05 versus WT.