Figure 2.

Binding of CG-1B on the surface of NDV. A, standard curves of NDV La Sota and F48E9 were established based on OD values of 2-fold serially diluted NDVs. B, binding of CG-1B to different NDV strains. NDV F48E9 and La Sota were added to 96-well plates coated with CG-1B (2 μg). β-Galactose or mannose was used as an antagonist. Bound virus particles were detected by ELISA with chicken anti-NDV La Sota antiserum. Uninfected chick embryo allantoic fluid and BSA were used as negative controls of NDV. The number of virions bound to CG-1B was calculated based on the linear standard curves. Mock-purified preparation-coated wells served as the negative control of CG-1B. Each value represents the mean and S.D. Data were compared by using Student's t test. ***, p < 0.001.