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. 2017 Oct 4;292(49):20141–20161. doi: 10.1074/jbc.M116.772897

Table 3.

Primers used for construction of the HN glycosylation site mutant cDNA

Primer name	Primer sequence (5′–3′)	Fragment region
LaFull-F	`TAGGCGCCATTATTGGCGGTGTG`	4896–8134^a
LaFull-R	`TGGCTTCTCTAACCCCGTCATC`	4896–8134^a
LaG1-F	`GGAGCTGCACAGAACAGTGGGT^b`	6757–6778
LaG1-R	`ACCCACTGTTCTGTGCAGCTCC`	6757–6778
LaG2-F	`AAGCGATACCAGGACACATGCCC`	7423–7445
LaG2-R	`GGGCATGTGTCCTGGTATCGCTT`	7423–7445
LaG3-F	`CCTATGACAGTCAGCCAGAAAAC`	7693–7715
LaG3-R	`GTTTTCTGGCTGACTGTCATAGG`	7693–7715
LaG4-F	`CTTCTATAGACAGCACACCTTGC`	7842–7864
LaG4-R	`GCAAGGTGTGCTGTCTATAGAAG`	7842–7864

^a Nucleotide positions of PCR-amplified fragment in La Sota are shown.

^b Letters underlined and italics represent mutated nucleotides, which changed the corresponding amino acids.