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Characterization of FplA lipase activity with multiple fluorescent substrates. A, enzymatic assays show that FplA is a PLA1-specific enzyme with no PLA2 activity. B, steady-state kinetics of multiple FplA constructs with 4-MuH. C, FplA(20–431) Kcat and Km values vary greatly across substrates with various acyl chain lengths. D and E, characterization of FplA enzyme kinetics.
