Figure 7.

Creation of an F. nucleatum 23726 ΔfplA. A, pDJSVT100 is a single-crossover integration plasmid for disruption of the fplA gene. Primers are labeled in red for PCRs A and B to confirm plasmid integration and gene knock-out. B, PCR confirmation of the F. nucleatum 23726 ΔfplA strain. C, analysis of FplA protein (85.6 kDa) in WT and ΔfplA by fluorescent chemical probe (ActivX TAMRA-FP) to label all active site serine enzymes in the bacteria (also serves as a load control), followed by transfer to PVDF for Western blot analysis by probing with an anti-FplA antibody.