Table 2.
Kd determination for LCAT binding to HDLs via BLI
| Variant | kon | koff | Kd | Sig.a | n |
|---|---|---|---|---|---|
| s−1 μm−1 | s−1 | μm | |||
| WT-peptide HDLs | 0.13 ± 0.02b | 0.065 ± 0.01 | 0.50 | NS | 3 |
| WT | 0.079 ± 0.005 | 0.057 ± 0.008 | 0.72 | 3 | |
| ΔNΔC | 0.011 ± 0.02 | 0.38 ± 0.1 | 35 | ** | 4 |
| W48A | 0.067 ± 0.02 | 0.27 ± 0.02 | 4.0 | ** | 3 |
| S181A | 0.064 ± 0.004 | 0.061 ± 0.01 | 0.95 | NS | 3 |
| T123I | 0.077 ± 0.005 | 0.070 ± 0.01 | 0.91 | ** | 3 |
| F382A | 0.088 ± 0.02 | 0.072 ± 0.02 | 0.82 | NS | 3 |
| I233A | 0.084 ± 0.01 | 0.22 ± 0.04 | 2.6 | * | 3 |
| M234A | 0.094 ± 0.005 | 0.091 ± 0.01 | 0.97 | ** | 3 |
| Δlid | 0.0044 ± 0.001 | 0.021 ± 0.004 | 4.8 | NS | 3 |
a Sig. represents significance for the Kd values determined individually for each replicate and then compared with WT using a two-tailed t test. NS indicates not significant. *, 0.01 < p < 0.05; **, 0.001 < p < 0.01.
b Error represents standard deviation.