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Denaturing and blue native gel electrophoresis of TbSTT3A-HA3. A and B, SDS-PAGE and anti-HA Western blotting (A) or blue native gel electrophoresis and anti-HA Western blotting (B) of anti-HA/protein G magnetic bead pull-outs from digitonin lysates of wild-type (lane 1) and in situ TbSTT3A-HA3-tagged (lane 2) bloodstream-form trypanosomes.
