Table 1.
Effect of aspartic acid on glycosite recognition by TbSTT3A
| Name | Sequence | pI | % EndoH-resistant (% STT3A transfer)a |
|---|---|---|---|
| Alanine control (Ala) | AAAAANATAAAAA | 6.01 | 6.8 ± 3.7 |
| D −5 | DAAAANATAAAAA | 3.10 | 34.4 ± 6.1 |
| D −4 | ADAAANATAAAAA | 3.10 | 44.8 ± 8.9 |
| D −3 | AADAANATAAAAA | 3.10 | 36.9 ± 2.6 |
| D −2 | AAADANATAAAAA | 3.10 | 36.5 ± 7.2 |
| D −1 | AAAADNATAAAAA | 3.10 | 70.5 ± 1.1 |
| D +1 | AAAAANDTAAAAA | 3.10 | 61.0 ± 11.3 |
| D +3 | AAAAANATDAAAA | 3.10 | 24.0 ± 2.7 |
| D +4 | AAAAANATADAAA | 3.10 | 16.4 ± 4.8 |
| D +5 | AAAAANATAADAA | 3.10 | 21.6 ± 4.4 |
| D +6 | AAAAANATAAADA | 3.10 | 27.2 ± 5.8 |
| D +7 | AAAAANATAAAAD | 3.10 | 10.7 ± 3.1 |
a The mean and standard deviations of the mean figures are based on n = 8 from two technical replicates of three biological replicates.