Table II. Top25 significantly differentially abundant proteins. Isolated primary AEC II from A. fumigatus-infected and mock-infected mice were analyzed using label-free quantitative proteomics. The protein list shows the Top25 proteins according to the Euclidian distance.
| UniProt Accession | Gene | Protein | ANOVA p value | Fold Changea | Mean +/− 95% CI Mock infected groupb | Mean +/− 95% CI A. fumigatus infected group2 | deuclc |
|---|---|---|---|---|---|---|---|
| O09046 | IL4I1 | l-amino-acid oxidase | 2.91E-10 | 42.94 | 8624.18 [+/− 5931.43] | 370356.39 [+/− 118323.65] | 10.97 |
| P11672 | Lcn2 | Neutrophil gelatinase-associated lipocalin | 1.08E-10 | 8.06 | 1031450.00 [+/− 285770.00] | 8311040.00 [+/− 1824460.00] | 10.41 |
| Q923B6 | Steap4 | Metalloreductase STEAP4 | 1.34E-08 | 6.92 | 66085.60 [+/− 21384.02] | 457432.17 [+/− 125652.65] | 8.35 |
| P50404 | Sftpd | Pulmonary surfactant-associated protein D | 1.07E-07 | 2.21 | 1975420.00 [+/− 330780.00] | 4360470.00 [+/− 545120.00] | 7.07 |
| P31725 | S100a9 | Protein S100-A9 | 4.78E-07 | 8.46 | 37955.95 [+/− 20097.61] | 321132.66 [+/− 98290.51] | 7.03 |
| P13597 | Icam1 | Intercellular adhesion molecule 1 | 2.06E-07 | 3.94 | 777314.87 [+/− 260005.13] | 3063350.00 [+/− 574190.00] | 6.97 |
| Q61646 | Hp | Haptoglobin | 2.58E-07 | 4.46 | 286190.30 [+/− 121723.63] | 1277260.00 [+/− 365220.00] | 6.93 |
| Q3TBT3 | Tmem173 | Stimulator of interferon genes protein | 2.92E-07 | 3.01 | 57301.22 [+/− 9673.70] | 172515.91 [+/− 35899.68] | 6.73 |
| Q78IQ7 | Slc39a4 | Zinc transporter ZIP4 | 1.58E-06 | 2.78 | 384125.49 [+/− 82143.61] | 1067720.00 [+/− 232640.00] | 5.99 |
| Q09200 | B4galnt1 | Beta-1,4 N-acetylgalactosaminyltransferase 1 | 1.98E-06 | 2.89 | 125044.33 [+/− 23328.29] | 361341.13 [+/− 96077.11] | 5.90 |
| Q61362 | Chi3l1 | Chitinase-3-like protein 1 | 2.25E-06 | 2.24 | 4970190.00 [+/− 844150.00] | 11157000.00 [+/− 2003300.00] | 5.77 |
| P27005 | S100a8 | Protein S100-A8 | 2.21E-05 | 5.43 | 242576.79 [+/− 92733.75] | 1316290.00 [+/− 823660.00] | 5.26 |
| A6X935 | Itih4 | Inter alpha-trypsin inhibitor, heavy chain 4 | 1.08E-05 | 2.82 | 131852.24 [+/− 25175.45] | 371453.29 [+/− 131722.70] | 5.19 |
| Q9R155 | Slc26a4 | Pendrin | 1.98E-05 | 3.41 | 38027.57 [+/− 20490.86] | 129805.62 [+/− 26243.77] | 5.03 |
| P21841 | Sftpc | Pulmonary surfactant-associated protein C | 1.26E-05 | 0.52 | 46854400.00 [+/− 8163400.00] | 24177000.00 [+/− 3882500.00] | 4.99 |
| Q61738 | Itga7 | Integrin alpha-7 | 3.40E-05 | 2.60 | 335197.85 [+/− 122747.89] | 872546.66 [+/− 109406.50] | 4.68 |
| O08917 | Flot1 | Flotillin-1 | 4.35E-05 | 1.90 | 161653.03 [+/− 40381.33] | 307831.45 [+/− 42515.77] | 4.46 |
| Q8BVZ5 | Il33 | Interleukin-33 | 5.08E-05 | 2.29 | 1361550.00 [+/− 205950.00] | 3112180.00 [+/− 1051480.00] | 4.46 |
| Q9EPR5 | Sorcs2 | VPS10 domain-containing receptor SorCS2 | 0.0002 | 4.77 | 15232.56 [+/− 11822.13] | 72633.77 [+/− 24979.04] | 4.30 |
| Q9JMA9 | Slc6a14 | Sodium- and chloride-dependent neutral and basic amino acid transporter B(0+) | 0.0002 | 2.60 | 44186.70 [+/− 23923.40] | 114773.00 [+/− 18011.89] | 3.88 |
| Q6PCP5 | Mff | Mitochondrial fission factor | 0.0002 | 1.90 | 91102.58 [+/− 24768.45] | 173528.95 [+/− 32773.39] | 3.84 |
| P04919 | Slc4a1 | Band 3 anion transport protein | 0.0008 | 4.45 | 197822.54 [+/− 286988.43] | 879608.93 [+/− 684861.07] | 3.77 |
| Q9QVP4 | Myl7 | Myosin regulatory light chain 2, atrial isoform | 0.0004 | 0.33 | 821689.24 [+/− 442970.76] | 270501.63 [+/− 90726.22] | 3.75 |
| B2RXS4 | Plxnb2 | Plexin-B2 | 0.0002 | 1.72 | 566770.22 [+/− 142301.89] | 972886.08 [+/− 104653.92] | 3.71 |
| P01027 | C3 | Complement C3 | 0.0005 | 3.10 | 1381810.00 [+/− 321080.00] | 4289030.00 [+/− 2433310.00] | 3.71 |
aAll displayed proteins were quantified with minimum two unique peptides. Ratios of means were calculated A. fumigatus infected/Mock infected.
bThe 95% confidence intervals were calculated using the normalized protein abundances. Displayed are the mean protein abundance and the difference to the upper and lower bound, respectively.
cThe protein list was ranked according to the Euclidian distance