Fig. 6.

Changes within the proteinaceous neighborhood of Asc1p. A, The bar chart depicts changes in the Asc1-BirA*p neighborhood in cells during glucose deprivation and heat stress (37 °C), and because of the R38D K40E exchanges relative to the neighborhood of Asc1-BirA*p during exponential growth. 19 proteins identified as Asc1p-neighbors during exponential growth are listed on the left. The bars represent log₂ SILAC ratios/enrichment values of the ASC1-birA*-strain starved from glucose (green), cultivated at 37 °C (red) or of the ASC1^DE-birA*-strain (blue). Proteins were considered as dynamic Asc1p-neighbors if log₂ SILAC ratios were outside −0.26 and 0.26. Stable Asc1p-neighbors, independent of the tested growth conditions or the DE-exchange, are listed at the top within the gray box (log₂ SILAC ratio between −0.26 and 0.26). Missing SILAC ratios are indicated by asterisks. Mean values that do not reach the respective threshold because of outlier values are indicated by triangles. B–D, Proteins specifically enriched in the Asc1p-neighborhood at (B) glucose starvation, (C) heat-stress, or (D) of the Asc1^DEp-variant were assigned to functional groups in a nonexclusive manner. Candidates of the respective groups are listed within the bar chart, those that are exclusively enriched in these specific experiments are listed in bold. The number of proteins per group is given by the x axis. Further proteins that do not belong to the chosen groups are listed below the diagrams.